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Advisory 

Virus free seed potato production through tissue culture

NAME OF ORGANIZATION: 

National Agricultural Research Centre (NARC)/PARC, Islamabad, Pakistan. 

CONTACT INSTITUTE/PROGRAMME: 
Tissue Culture Section, Agricultural Biotechnology Institute (ABI), NARC. 

LOCATION OF APPLICATION: 

Northern Areas of Pakistan. 

KEY CHARACTERISTICS OF THE TECHNOLOGY:

To get higher yields.
Reduce dependence of imports.
Increase the income of farmers.
Market the technology through private sector.

PRODUCTION GUIDELINES (IF APPLICABLE): 

Raise virus free potato clones through meristem tip culture.
In vitro cloning.
Screening against viruses through ELISA.
Multiplication of clean stock.
Raising of mother plants in glass houses.
Virus testing by Federal Seed Certification Deptt. (FSCD).
Rapid multiplication.
Transportation of rooted microcuttings to Northern Areas.
Establishment of potato fields at farmers premises.
Production of pre-basic seed and its storage in Northern Areas.
Multiplication for pre-basic-II Storage.
Multiplication for basic-II, Storage.
Multiplication for certified seed.
Marketing by the Private Sector.
ABI supplies the source material, whereas all other inputs are by the private sector.

COST BENEFIT RATIO: 

1:3 

RISK INVOLVEMENT IN ADOPTING THE TECHNOLOGY (BIOPHYSICAL, SOCIAL & ECONOMIC): 

Inclement weather conditions
Non availability of fertilizer at the appropriate time.

DEGREE OF ADOPTION: 

The technology is acceptable by farmers and the private sector. 

IMPACT ON ENVIRONMENT: 

No adverse effect on environment. 

SUSTAINABILITY OF TECHNOLOGY (BIOPHYSICAL, SOCIAL AND ECONOMIC): 

Sustainable. 

EXPECTED OUTCOME: 

Availability of virus free potato seed of indigenous clones.
Higher return to farmers.
Lessening of imports.

RECOMMENDATIONS:

Pre-basic and basic seed of potato must be produced in high hills.
At least 6 multiplications till the certified level must be undertaken in aphid free zones.
Transportation of certified seed must be from high hills to plains and never vice-versa.


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